Instrument: Ion Torrent Proton
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Total RNAs were extracted as described previously (Mizutani et al., 2011, J Biol Chem). Libraries were prepared using Dynabeads mRNA DIRECT Purification Kit and Ion Total RNA-seq kit v2 (Thermo Fisher Scientific). Sequencing was performed as described (Sakurai et al., 2016, Oncogene).